COX2/Cyclooxygenase 2 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
prostaglandin-endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human COX2(26-41aa CQNRGVCMSVGFDQYK), different from the related rat sequence by two amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	PTGS2
Protein	Prostaglandin G/H synthase 2
Uniprot ID	P35354
Function	Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Tissue Specificity
Sub-cellular localization	Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
Sequence Similarities	Belongs to the prostaglandin G/H synthase family.
Aliases	COX 2 antibody|COX-2 antibody|COX2 antibody|Cyclooxygenase 2 antibody|Cyclooxygenase 2b antibody|Cyclooxygenase antibody| Cyclooxygenase-2 antibody|Cyclooxygenase2 antibody|EC 1.14.99.1 antibody|fj02a10 antibody|Glucocorticoid-regulated inflammatory cyclooxygenase antibody|Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody|GRIPGHS antibody|hCox 2 antibody|Macrophage activation-associated marker protein P71/73 antibody|OTTHUMP00000033524 antibody|PES-2 antibody|PGG/HS antibody|PGH synthase 2 antibody|PGH2_HUMAN antibody|PGHS 2 antibody|PGHS-2 antibody|PGHS2 antibody|PHS 2 antibody|PHS II antibody|PHS2 antibody|Prostaglandin endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase) antibody|Prostaglandin endoperoxide synthase 2 antibody|Prostaglandin G/H synthase 2 antibody|Prostaglandin G/H synthase 2 precursor antibody|Prostaglandin G/H synthase and cyclooxygenase antibody|Prostaglandin G/H synthase antibody|Prostaglandin H2 synthase 2 antibody|prostaglandin-endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase antibody|Prostaglandin-endoperoxide synthase 2 antibody|PTGS2 antibody|ptgs2a antibody|TIS10 antibody|TIS10 protein antibody|unp1239 antibody|wu:fj02a10 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information