COX1/Cyclooxygenase 1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
prostaglandin-endoperoxide synthase 1(prostaglandin G/H synthase and cyclooxygenase)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human COX1(35-52aa CCYYPCQHQGICVRFGLD), different from the related rat and mouse sequences by two amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	PTGS1
Protein	Prostaglandin G/H synthase 1
Uniprot ID	P23219
Function	Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
Tissue Specificity
Sub-cellular localization	Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
Sequence Similarities	Belongs to the prostaglandin G/H synthase family.
Aliases	COX 1 antibody|COX 3 antibody|COX-1 antibody|COX1 antibody|COX3 antibody|Cyclooxygenase 1 antibody|Cyclooxygenase 3, included antibody|Cyclooxygenase-1 antibody|Cyclooxygenase1 antibody|EC 1.14.99.1 antibody|Partial COX1 proteins, included antibody|PCOX 1 antibody|PCOX1 antibody|PGG/HS antibody|PGH synthase 1 antibody|PGH1_HUMAN antibody|PGHS 1 antibody|PGHS-1 antibody|PGHS1 antibody|PHS 1 antibody|PHS1 antibody|Prostaglandin endoperoxide synthase 1 antibody|Prostaglandin G/H synthase 1 antibody|Prostaglandin G/H synthase and cyclooxygenase antibody|Prostaglandin H Synthase 1 antibody|Prostaglandin H2 synthase 1 antibody|Prostaglandin-endoperoxide synthase 1(prostaglandin G/H synthase and cyclooxygenase) antibody|Prostaglandin-endoperoxide synthase 1 antibody|PTGHS antibody|PTGS 1 antibody|PTGS1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information