More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| gap junction protein, alpha 1, 43kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(351-367aa HELQPLAIVDQRPSSRA), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GJA1 |
Protein | Gap junction alpha-1 protein |
Uniprot ID | P17302 |
Function | Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). |
Tissue Specificity | Expressed in the heart and fetal cochlea. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the connexin family. Alpha-type (group II) subfamily. |
Aliases | Connexin 43 antibody|Connexin-43 antibody|Connexin43 antibody|Cx 43 antibody|CX43 antibody|CXA1_HUMAN antibody|DFNB38 antibody|Gap junction 43 kDa heart protein antibody|Gap junction alpha 1 protein antibody|Gap junction alpha-1 protein antibody|Gap junction protein alpha 1 43kD antibody|Gap junction protein alpha 1 43kDa(connexin 43) antibody|Gap junction protein alpha 1 43kDa antibody|Gap junction protein alpha like antibody|GJA 1 antibody|Gja1 antibody|GJAL antibody|HGNC:4282 antibody|HGNC:8112 antibody|Oculodentodigital dysplasia antibody|ODD antibody|ODDD antibody|ODOD antibody|SDTY 3 antibody|SDTY3 antibody|Syndactyly type III antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Connexin 43/GJA1 antibody, ASA-B0484, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-Connexin 43/GJA1 antibody, ASA-B0484, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
Anti-Connexin 43/GJA1 antibody, ASA-B0484, IHC(F)
IHC(F): Rat Cardiac Muscle Tissue
IHC(F): Rat Cardiac Muscle Tissue