More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| gap junction protein, alpha 5, 40kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 40/GJA5(341-358aa KRRLSKASSKARSDDLSV), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GJA5 |
Protein | Gap junction alpha-5 protein |
Uniprot ID | P36382 |
Function | One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
Sequence Similarities | |
Aliases | Connexin 40 antibody|Connexin-40 antibody|Cx40 antibody|CXA5_HUMAN antibody|Gap junction alpha 5 protein antibody|Gap junction alpha-5 protein antibody|Gap junction protein alpha 5 40kD(connexin 40) antibody|Gap junction protein alpha 5 antibody|GJA5 antibody|MGC11185 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Connexin 40/GJA5 antibody, ASA-B0483, Western blotting
Lane 1: Mouse Heart Tissue Lysate
Lane 2: Mouse Heart Tissue Lysate
Lane 1: Mouse Heart Tissue Lysate
Lane 2: Mouse Heart Tissue Lysate
Anti-Connexin 40/GJA5 antibody, ASA-B0483, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
