More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| gap junction protein, beta 1, 32kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Connexin 32/GJB1(215-231aa RACARRAQRRSNPPSRK), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GJB1 |
Protein | Gap junction beta-1 protein |
Uniprot ID | P08034 |
Function | One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
Sequence Similarities | Belongs to the connexin family. Beta-type (group I) subfamily. |
Aliases | Charcot Marie Tooth neuropathy X linked antibody|CMTX 1 antibody|CMTX antibody|CMTX1 antibody|Connexin-32 antibody|Connexin32 antibody|CX 32 antibody|CX32 antibody|CXB1_HUMAN antibody|GAP junction 28 kDa liver protein antibody|Gap junction beta 1 protein antibody|Gap junction beta-1 protein antibody|Gap junction protein beta 1 32kD antibody|Gap junction protein beta 1 antibody|GJB 1 antibody|GJB1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Connexin 32/GJB1 antibody, ASA-B0481, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Cardiac Muscle Tissue Lysate
Lane 3: Rat Skeletal Muscle Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 9: COLO320 Cell Lysate
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Cardiac Muscle Tissue Lysate
Lane 3: Rat Skeletal Muscle Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 9: COLO320 Cell Lysate
Anti-Connexin 32/GJB1 antibody, ASA-B0481, IHC(P)
IHC(P):Rat Kidney Tissue
IHC(P):Rat Kidney Tissue
