ASA-B0477
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Collagen, Type III Antibody (monoclonal)
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Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
collagen, type III, alpha 1 | Monoclonal | IgG | Mouse | Human, Rat | IHC-F, WB | Ascites |
Immunogen | ||||||
Human collagen type III. |
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Gene | COL3A1 |
Protein | Collagen alpha-1(III) chain |
Uniprot ID | Human(P02461), Mouse(P08121), Rat(P13941) |
Function | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Belongs to the fibrillar collagen family. |
Aliases | EDS4A|COLLAGEN, FETAL|COLLAGEN, BLOOD VESSEL |
Application | Concentration* | Species | Validated Using** |
Western blot | 0.25-0.5μg/ml | Human, rat | AssaySolutio's ECL kit |
Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, rat | AssaySolutio's IHC/ICC Detection kit |