More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| collagen, type II, alpha 1 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | COL2A1 |
Protein | Collagen alpha-1(II) chain |
Uniprot ID | P28481 |
Function | Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Belongs to the fibrillar collagen family. |
Aliases | Alpha 1 type II collagen antibody|Alpha-1 type II collagen antibody|AOM antibody|Cartilage collagen antibody|Chondrocalcin antibody|CO2A1_HUMAN antibody|COL11A3 antibody|COL2A1 antibody|COL2A1 protein antibody|Collagen alpha 1(II) chain precursor antibody|Collagen II alpha 1 polypeptide antibody|Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital) antibody|MGC131516 antibody|SEDC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Collagen II antibody, ASA-B0470, IHC(F)
IHC(F): Rat Trachea Tissue
IHC(F): Rat Trachea Tissue
Anti-Collagen II antibody, ASA-B0470, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 1: Rat Heart Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
