More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| collagen, type I, alpha 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen I(1203-1218aa QPPQEKAHDGGRYYRA), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | COL1A1 |
Protein | Collagen alpha-1(I) chain |
Uniprot ID | P02452 |
Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
Tissue Specificity | Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Belongs to the fibrillar collagen family. |
Aliases | Alpha 1 type I collagen antibody|Alpha 2 type I collagen antibody|Alpha-1 type I collagen antibody|CO1A1_HUMAN antibody|COL1A1 antibody|COL1A2 antibody|Collagen alpha-1(I) chain antibody|Collagen I alpha 1 polypeptide antibody|Collagen I alpha 2 polypeptide antibody|Collagen Of Skin Tendon And Bone antibody|Collagen Type 1 antibody|Collagen type I alpha 1 antibody|Collagen type I alpha 2 antibody|OI4 antibody|Osteogenesis Imperfecta Type IV antibody|Pro alpha 1(I) collagen antibody|Type I procollagen antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Mouse Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Collagen I antibody, ASA-B0468, Western blotting
Lane 1: Rat Skeletal Muscles Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 1: Rat Skeletal Muscles Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Anti-Collagen I antibody, ASA-B0468, IHC(F)
IHC(F): Human Placenta Tissue
IHC(F): Human Placenta Tissue
Anti-Collagen I antibody, ASA-B0468, ICC
ICC: NIH3T3 Cell
ICC: NIH3T3 Cell