CNTF Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
ciliary neurotrophic factor	Polyclonal	IgG	Rabbit	Human, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
E.coli-derived human CNTF recombinant protein (Position: A2-M200). Human CNTF shares 83% and 84% amino acid (aa) sequences identity with mouse and rat CNTF, respectively.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CNTF
Protein	Ciliary neurotrophic factor
Uniprot ID	P26441
Function	CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Tissue Specificity	Nervous system.
Sub-cellular localization	Cytoplasm.
Sequence Similarities	Belongs to the CNTF family.
Aliases	Ciliary Neurotrophic Factor antibody|CNTF antibody|CNTF_HUMAN antibody|HCNTF antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information