More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| v-myc myelocytomatosis viral oncogene homolog(avian) | Monoclonal | IgG | Mouse | Human | IHC-P, ICC, WB | Ascites |
Immunogen |
| Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MYC |
Protein | Myc proto-oncogene protein |
Uniprot ID | Human(P01106), Mouse(P01108), Rat(P09416) |
Function | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. |
Tissue Specificity | |
Sub-cellular localization | Nucleus, nucleoplasm. |
Sequence Similarities | Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases | C-Myc|bHLHe39|Proto-oncogene c-Myc|Transcription factor p64|Class E basic helix-loop-helix protein 39 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 4μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 8μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | Suitable | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-c-Myc antibody (monoclonal), ASA-B0251, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
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