More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chymase 1, mast cell | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CMA1 |
Protein | Chymase |
Uniprot ID | P23946 |
Function | Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. |
Tissue Specificity | Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. |
Sub-cellular localization | Secreted. Cytoplasmic granule. Note: Mast cell granules. |
Sequence Similarities | Belongs to the peptidase S1 family. Granzyme subfamily. |
Aliases | Alpha-chymase antibody|Chymase 1 antibody|Chymase 1 mast cell antibody|Chymase antibody|Chymase, heart antibody|Chymase, mast cell antibody|CMA1 antibody|CMA1_HUMAN antibody|CYH antibody|CYM antibody|EC 3.4.21.39 antibody|Mast cell chymase 1 antibody|Mast cell protease 3 antibody|Mast cell protease 5 antibody|Mast cell protease I antibody|Mast cell protease III antibody|Mcp-5 antibody|MCP3P antibody|Mcpt5 antibody|MGC119890 antibody|MGC119891 antibody|MMCP-5 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CMA1 antibody, ASA-B0462--1.jpg
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-CMA1 antibody, ASA-B0462--2.jpg
All lanes: Anti-CMA1(ASA-B0462) at 0.5ug/ml
WB: Recombinant Human CMA1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti-CMA1(ASA-B0462) at 0.5ug/ml
WB: Recombinant Human CMA1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
