More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| claudin 2 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CLDN2 |
Protein | Claudin-2 |
Uniprot ID | P57739 |
Function | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. |
Tissue Specificity | |
Sub-cellular localization | Cell junction, tight junction . Cell membrane ; Multi-pass membrane protein . |
Sequence Similarities | |
Aliases | Claudin-2 antibody|Claudin2 antibody|CLD2_HUMAN antibody|CLDN 2 antibody|CLDN2 antibody|OTTHUMP00000023793 antibody|SP 82 antibody|SP82 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Claudin 2 antibody, ASA-B0459, Western blotting
All lanes: Anti Claudin 2 (ASA-B0459) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 25KD
Observed bind size: 25KD
All lanes: Anti Claudin 2 (ASA-B0459) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 25KD
Observed bind size: 25KD
Anti- Claudin 2 antibody, ASA-B0459, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
