Claudin 2 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
claudin 2	Polyclonal	IgG	Rabbit	Human, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CLDN2
Protein	Claudin-2
Uniprot ID	P57739
Function	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity.
Tissue Specificity
Sub-cellular localization	Cell junction, tight junction . Cell membrane ; Multi-pass membrane protein .
Sequence Similarities
Aliases	Claudin-2 antibody|Claudin2 antibody|CLD2_HUMAN antibody|CLDN 2 antibody|CLDN2 antibody|OTTHUMP00000023793 antibody|SP 82 antibody|SP82 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Rat Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information