More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chromogranin A (parathyroid secretory protein 1) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Chromogranin A recombinant protein (Position: K321-G457). Human Chromogranin A shares 80% and 82% amino acid (aa) sequences identity with mouse and rat Chromogranin A, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CHGA |
Protein | Chromogranin-A |
Uniprot ID | P10645 |
Function | Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. |
Tissue Specificity | GE-25 is found in the brain. |
Sub-cellular localization | Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle membrane . Secreted. Note: Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. |
Sequence Similarities | |
Aliases | beta Granin antibody|CGA antibody|CHG A antibody|CHGA antibody|Chromogranin A parathyroid secretory protein 1 antibody|Chromogranin A precursor antibody|ChromograninA antibody|CMGA_HUMAN antibody|ER-37 antibody|Pancreastatin antibody|Parastatin antibody|Parathyroid secretory protein 1 antibody|Pituitary secretory protein I antibody|SP I antibody|SP-I antibody|SP1 antibody|SPI antibody|Vasostatin antibody|Vasostatin I antibody|Vasostatin II antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Chromogranin A antibody, ASA-B0453--1.jpg
All lanes: Anti Chromogranin A (ASA-B0453) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Gaster Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: PC-12 Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD
All lanes: Anti Chromogranin A (ASA-B0453) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Gaster Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: PC-12 Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD
Anti-Chromogranin A antibody, ASA-B0453--2.jpg
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti-Chromogranin A antibody, ASA-B0453--3.jpg
IHC(P): Rat Paranephros Tissue
IHC(P): Rat Paranephros Tissue