More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cystic fibrosis transmembrane conductance regulator(ATP-binding cassette sub-family C, member 7) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CFTR(1463-1480aa QIAALKEETEEEVQDTRL), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CFTR |
Protein | Cystic fibrosis transmembrane conductance regulator |
Uniprot ID | P13569 |
Function | Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. |
Tissue Specificity | Expressed in the respiratory airway, including bronchial epithelium, and in the female reproductive tract, including oviduct (at protein level). |
Sub-cellular localization | Early endosome membrane; Multi-pass membrane protein. Cell membrane. Note: In the oviduct and bronchus, detected on the apical side of epithelial cells, but not associated with cilia. |
Sequence Similarities | Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily. |
Aliases | ABC 35 antibody|ABC35 antibody|ABCC 7 antibody|ABCC7 antibody|ATP binding cassette sub family C member 7 antibody|ATP Binding Cassette Superfamily C Member 7 antibody|ATP binding cassette transporter sub family C member 7 antibody|ATP-binding cassette sub-family C member 7 antibody|cAMP dependent chloride channel antibody|cAMP-dependent chloride channel antibody|CF antibody|CFTR antibody|CFTR/MRP antibody|CFTR_HUMAN antibody|Channel conductance controlling ATPase antibody|Channel conductance-controlling ATPase antibody|Cystic fibrosis transmembrane conductance regulator antibody|Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 antibody|Cystic Fibrosis Transmembrane Regulator antibody|dJ760C5.1 antibody|MRP 7 antibody|MRP7 antibody|TNR CFTR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CFTR antibody, ASA-B0449, Western blotting
WB: Rat Pancreas Tissue Lysate
WB: Rat Pancreas Tissue Lysate
Anti-CFTR antibody, ASA-B0449, IHC(P)
IHC(P): Human Pancreatic Cancer Tissue
IHC(P): Human Pancreatic Cancer Tissue
