More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cell division cycle 6 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Cdc6 recombinant protein (Position: V147-A549). Human Cdc6 shares 87.3% amino acid (aa) sequence identity with mouse Cdc6. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDC6 |
Protein | Cell division control protein 6 homolog |
Uniprot ID | Q99741 |
Function | Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the CDC6/cdc18 family. |
Aliases | Cdc 18L antibody|Cdc 6 antibody|CDC18 (cell division cycle 18, S.pombe, homolog) like antibody|CDC18 (S.pombe) antibody|Cdc18 related protein antibody|CDC18(S.pombe) antibody| Cdc18-related protein antibody|Cdc18L antibody|cdc6 antibody|CDC6 cell division cycle 6 homolog antibody|CDC6 related protein antibody|CDC6-related protein antibody| CDC6_HUMAN antibody| Cdc6p antibody|CDC-6 antibody|Cell Cycle Controller antibody|CELL CYCLE CONTROLLER CDC6 antibody| Cell division control protein 6 antibody|Cell division control protein 6 homolog antibody| Cell Division Cycle 18 antibody|Cell division cycle 6 homolog (S. cerevisiae) antibody|Cell division cycle 6 homolog antibody|HsCDC 6 antibody|HsCDC18 antibody|HsCDC6 antibody|p62 antibody|p62(cdc 6) antibody|p62(cdc6) antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Cdc6 antibody, ASA-B0426, Western blotting
All lanes: Anti Cdc6 (ASA-B0426) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 63KD
Observed bind size: 63KD
All lanes: Anti Cdc6 (ASA-B0426) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 63KD
Observed bind size: 63KD
Anti- Cdc6 antibody, ASA-B0426, IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue
Anti- Cdc6 antibody, ASA-B0426, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue