More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cell division cycle 42(GTP binding protein, 25kDa) | Polyclonal | IgG | Rabbit | Bovine, Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human CDC42(121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDC42 |
Protein | Cell division control protein 42 homolog(Cdc42 homolog) |
Uniprot ID | P60953 |
Function | Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Note: Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome. |
Sequence Similarities | Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. |
Aliases | CDC 42 antibody|CDC42 antibody|CDC42_HUMAN antibody|CDC42Hs antibody|Cell division control protein 42 homolog antibody|Cell division cycle 42(GTP binding protein 25kDa) antibody|Cell division cycle 42 antibody|Cell division cycle 42 isoform 1 antibody|Cell division cycle 42 isoform 2 antibody|dJ224A6.1.1(cell division cycle 42(GTP-binding protein, 25kD)) antibody|dJ224A6.1.1 antibody|dJ224A6.1.2(cell division cycle 42(GTP-binding protein, 25kD)) antibody|dJ224A6.1.2 antibody|G25K antibody|G25K GTP binding protein antibody|G25K GTP-binding protein antibody|Growth regulating protein antibody|GTP binding protein 25kD antibody|GTP binding protein 25kDa antibody|Small GTP binding protein CDC42 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Bovine, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CDC42 antibody, ASA-B0422, Western blotting
Lane 1: Recombinant Human CDC42 Protein 10ng
Lane 2: Recombinant Human CDC42 Protein 5ng
Lane 3: Recombinant Human CDC42 Protein 2.5ng
Lane 1: Recombinant Human CDC42 Protein 10ng
Lane 2: Recombinant Human CDC42 Protein 5ng
Lane 3: Recombinant Human CDC42 Protein 2.5ng
Anti-CDC42 antibody, ASA-B0422, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
