More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cell division cycle 34 homolog(S. cerevisiae) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Cdc34(148-163aa YRKWKESKGKDREYTD), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDC34 |
Protein | Ubiquitin-conjugating enzyme E2 R1 |
Uniprot ID | P49427 |
Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes. |
Tissue Specificity | Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. |
Sub-cellular localization | Cytoplasm. Nucleus. Note: The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase. |
Sequence Similarities | Belongs to the ubiquitin-conjugating enzyme family. |
Aliases | Cdc 34 antibody|Cdc34 antibody|Cell division cycle 34 antibody|Cell division cycle 34 homolog(S. cerevisiae) antibody|Cell division cycle 34 homolog antibody|E2 CDC34 antibody|UB2R1_HUMAN antibody|UBC 3 antibody|UBC3 antibody|UBCH3 antibody|UBE2 R1 antibody|UBE2R1 antibody|Ubiquitin carrier protein antibody|Ubiquitin conjugating enzyme Cdc34 antibody|Ubiquitin conjugating enzyme E2 32 kDa complementing antibody|Ubiquitin protein ligase antibody|Ubiquitin protein ligase R1 antibody|Ubiquitin-conjugating enzyme E2 R1 antibody|Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody|Ubiquitin-conjugating enzyme E2-CDC34 antibody|Ubiquitin-protein ligase R1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cdc34 antibody, ASA-B0419, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: NIH/3T3 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: A549 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: NIH/3T3 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: A549 Cell Lysate
Anti-Cdc34 antibody, ASA-B0419, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
