Cdc25B Antibody View larger

Cdc25B Antibody

ASA-B0416

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

cell division cycle 25 homolog B(S. pombe) Polyclonal IgG Rabbit Human, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc25B(541-559aa DYRPMNHEAFKDELKTFRL), different from the related mouse sequence by three amino acids.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

CDC25B

Protein

M-phase inducer phosphatase 2

Uniprot ID

P30305

Function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Tissue Specificity

Sub-cellular localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.

Sequence Similarities

Belongs to the MPI phosphatase family.

Aliases

AI604853 antibody|Cdc 25B antibody|Cdc25b antibody|CDC25B1 antibody|CDC25B2 antibody|CDC25B3 antibody|CDC25HU2 antibody|Cdc25m2 antibody|Cell division cycle 25 homolog B antibody|Cell division cycle 25B antibody|Cell division cycle 25B isoform 1 antibody|Cell division cycle 25B isoform 2 antibody|Cell division cycle 25B isoform 3 antibody|Cell division cycle 25B isoform 4 antibody|Dual specificity phosphatase Cdc25B antibody|EC 3.1.3.48 antibody|M phase inducer phosphatase 2 antibody|M-phase inducer phosphatase 2 antibody|MPIP2_HUMAN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Rat HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cdc25B antibody, ASA-B0416, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Anti-Cdc25B antibody, ASA-B0416, IHC(P)
IHC(P): Human Lung Cancer Tissue

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