More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cell division cycle 25 homolog B (S. pombe) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Cdc25B recombinant protein (Position: M119-L248). Human Cdc25B shares 71% and 68.2% amino acid (aa) sequence identity with mouse and rat Cdc25B, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDC25B |
Protein | M-phase inducer phosphatase 2 |
Uniprot ID | P30305 |
Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. |
Sequence Similarities | Belongs to the MPI phosphatase family. |
Aliases | AI604853 antibody|Cdc 25B antibody|Cdc25b antibody|CDC25B1 antibody| CDC25B2 antibody| CDC25B3 antibody|CDC25HU2 antibody|Cdc25m2 antibody| Cell division cycle 25 homolog B antibody|Cell division cycle 25B antibody|Cell division cycle 25B isoform 1 antibody|Cell division cycle 25B isoform 2 antibody| Cell division cycle 25B isoform 3 antibody|Cell division cycle 25B isoform 4 antibody|Dual specificity phosphatase Cdc25B antibody|EC 3.1.3.48 antibody|M phase inducer phosphatase 2 antibody|M-phase inducer phosphatase 2 antibody|MPIP2_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Cdc25B antibody, ASA-B0417, Western blotting
All lanes: Anti Cdc25B (ASA-B0417) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Predicted bind size: 65KD
Observed bind size: 65KD
All lanes: Anti Cdc25B (ASA-B0417) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Predicted bind size: 65KD
Observed bind size: 65KD
Anti- Cdc25B antibody, ASA-B0417, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- Cdc25B antibody, ASA-B0417, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue