More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cell division cycle 25 homolog A(S. pombe) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Cdc25A(1-17aa MELGPEPPHRRRLLFAC), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDC25A |
Protein | M-phase inducer phosphatase 1 |
Uniprot ID | P30304 |
Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the MPI phosphatase family. |
Aliases | Cdc 25a antibody|CDC25A antibody|CDC25A1 antibody|CDC25A2 antibody|CDC25A2 CAG isoform antibody|Cell division cycle 25 homolog A(S. pombe) antibody|Cell division cycle 25A antibody|Cell division cycle 25A isoform a antibody|Cell division cycle 25A isoform b antibody|D9Ertd393e antibody|Dual specificity phosphatase Cdc25A antibody|M phase inducer phosphatase 1 antibody|M-phase inducer phosphatase 1 antibody|MGC115549 antibody|MPIP1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cdc25A antibody, ASA-B0415, Western blotting
All lanes: Anti Cdc25A (ASA-B0415) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 70KD
All lanes: Anti Cdc25A (ASA-B0415) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 70KD
Anti-Cdc25A antibody, ASA-B0415, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
