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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD82 molecule | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CD82 recombinant protein (Position: A98-Y267). Human CD82 shares 69% and 67% amino acid (aa) sequences identity with mouse and rat CD82, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD82 |
Protein | CD82 antigen |
Uniprot ID | P27701 |
Function | Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. |
Tissue Specificity | Lymphoid specific. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the tetraspanin (TM4SF) family. |
Aliases | 4F9 antibody|Antigen detected by monoclonal and antibody IA4 antibody|C33 antibody|C33 antigen antibody|CD 82 antibody|CD82 antibody|Cd82 antibody|CD82 antigen antibody|CD82 molecule antibody|CD82_HUMAN antibody|GR 15 antibody|GR15 antibody|IA 4 antibody|IA4 antibody|Inducible membrane protein antibody|Inducible membrane protein R2 antibody|KAI 1 antibody|KAI1 antibody|Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody|Kangai 1 antibody|Kangai1 antibody|Leukocyte surface antigen R2 antibody|Metastasis suppressor Kangai 1 antibody|Metastasis suppressor Kangai-1 antibody|Metastasis suppressor Kangai1 antibody|Prostate cancer antimetastasis gene KAI1 antibody|R2 antibody|R2 leukocyte antigen antibody|SAR 2 antibody|SAR2 antibody|ST 6 antibody|ST6 antibody|Suppression of tumorigenicity 6 antibody|Suppression of tumorigenicity 6 prostate antibody|Suppressor of tumorigenicity 6 antibody|Suppressor of tumorigenicity 6 protein antibody|Tetraspanin 27 antibody|Tetraspanin-27 antibody|Tetraspanin27 antibody|Tspan 27 antibody|Tspan-27 antibody|Tspan27 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD82 antibody, ASA-B0408, Western blotting
All lanes: Anti CD82 (ASA-B0408) at 0.5ug/ml
WB: Recombinant Human CD82 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti CD82 (ASA-B0408) at 0.5ug/ml
WB: Recombinant Human CD82 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- CD82 antibody, ASA-B0408, Western blotting
All lanes: Anti CD82 (ASA-B0408) at 0.5ug/ml
Lane 1: HL-60 Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Lane 4: U937 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
All lanes: Anti CD82 (ASA-B0408) at 0.5ug/ml
Lane 1: HL-60 Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Lane 4: U937 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
Anti-CD82 antibody, ASA-B0408, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue