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CD8 alpha Antibody

ASA-B0403

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
CD8a molecule Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CD8A

Protein

 T-cell surface glycoprotein CD8 alpha chain

Uniprot ID

 P01732

Function

 Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Tissue Specificity

Sub-cellular localization

 Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

 Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Aliases

 alpha polypeptide (p32) antibody|CD 8a antibody|CD8 alpha chain antibody|CD8 antibody|CD8 antigen alpha chain antibody|CD8 antigen alpha polypeptide antibody|CD8 antigen alpha polypeptide (p32) antibody|CD8a antibody|CD8a antigen antibody|CD8a molecule antibody|CD8A_HUMAN antibody|Leu 2 antibody|Leu2 antibody|Leu2 T lymphocyte antigen antibody|Ly3 antibody|LYT3 antibody|MAL antibody|OKT8 T cell antigen antibody|OTTHUMP00000160760 antibody|OTTHUMP00000160764 antibody|OTTHUMP00000203528 antibody|OTTHUMP00000203721 antibody|p32 antibody|T cell antigen Leu2 antibody|T cell co receptor antibody|T cell coreceptor antibody|T cell surface glycoprotein CD8 alpha chain antibody|T lymphocyte differentiation antigen T8/Leu 2 antibody|T lymphocyte differentiation antigen T8/Leu2 antibody|T-cell surface glycoprotein CD8 alpha chain antibody|T-lymphocyte differentiation antigen T8/Leu-2 antibody|T8 T cell antigen antibody|T8/Leu-2 T-lymphocyte differentiation antigen antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD 8 alpha antibody, ASA-B0403, Western blotting
All lanes: Anti CD 8 alpha (ASA-B0403) at 0.5ug/ml
WB: Recombinant Human CD 8 alpha Protein 0.5ng
Predicted bind size: 29KD
Observed bind size: 29KD
Anti- CD 8 alpha antibody, ASA-B0403, Western blotting
All lanes: Anti CD 8 alpha (ASA-B0403) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Lane 3: HL-60 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 55KD
Anti- CD 8 alpha antibody, ASA-B0403, IHC(P)
IHC(P): Human Tonsil Tissue

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