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CD79b Antibody

ASA-B0402

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
CD79b molecule, immunoglobulin-associated beta Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

E.coli-derived human CD79b recombinant protein (Position: A29-E229). Human CD79b shares 70% amino acid (aa) sequence identity with mouse CD79b.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CD79B

Protein

 B-cell antigen receptor complex-associated protein beta chain

Uniprot ID

 P40259

Function

 Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

Tissue Specificity

B-cells.

Sub-cellular localization

 Cell membrane; Single-pass type I membrane protein. Note: Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.

Sequence Similarities

 Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Aliases

 AGM6 antibody|B cell antigen receptor complex associated protein beta chain antibody|B cell specific glycoprotein B29 antibody|B-cell antigen receptor complex-associated protein beta chain antibody|B-cell-specific glycoprotein B29 antibody|B29 antibody|B29/Ig-beta/CD79b antibody|CD 79b antibody|CD79b antibody|CD79b antigen (immunoglobulin associated beta) antibody|CD79b antigen antibody|CD79b molecule antibody|CD79b molecule immunoglobulin associated beta antibody|CD79b protein antibody|CD79B_HUMAN antibody|Ig beta antibody|Ig-beta antibody|IGB antibody|Igbeta antibody|Immunoglobulin associated B29 antibody|Immunoglobulin associated B29 protein antibody|Immunoglobulin associated beta antibody|Immunoglobulin associated protein antibody|Immunoglobulin-associated B29 protein antibody|MGC108607 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, RatAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD79B antibody, ASA-B0402, Western blotting
All lanes: Anti CD79B (ASA-B0402) at 0.5ug/ml
WB: Recombinant Human CD79B Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- CD79B antibody, ASA-B0402, Western blotting
All lanes: Anti CD79b (ASA-B0402) at 0.5ug/ml
Lane 1: Raji Whole Cell Lysate at 40ug
Lane 2: Hl-60 Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 35KD
Anti- CD79B antibody, ASA-B0402,IHC(P)
IHC(P): Mouse Spleen Tissue

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