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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD79a molecule, immunoglobulin-associated alpha | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CD79a recombinant protein (Position: T121-P226). Human CD79a shares 91% amino acid (aa) sequence identity with mouse CD79a. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD79A |
Protein | B-cell antigen receptor complex-associated protein alpha chain |
Uniprot ID | P11912 |
Function | Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src- family tyrosine kinases. Represses BCR signaling during development of immature B-cells. |
Tissue Specificity | B-cells. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Note: Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. |
Sequence Similarities | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
Aliases | B cell antigen receptor complex associated protein alpha chain antibody|B cell antigen receptor complex associated protein alpha chain precursor antibody|B Lymphocyte Specific MB1 Protein antibody|B lymphocyte-specific MB1 protein antibody|B-cell antigen receptor complex-associated protein alpha chain antibody|CD 79a antibody|CD79a antibody|CD79a antigen (immunoglobulin-associated alpha) antibody|CD79A antigen antibody|CD79A antigen immunoglobulin associated alpha antibody|CD79a molecule immunoglobulin associated alpha antibody|CD79a molecule, immunoglobulin-associated alpha antibody|CD79A_HUMAN antibody|Ig alpha antibody|Ig-alpha antibody|IGA antibody|IgM-alpha antibody|Immunoglobulin Associated Alpha antibody|Immunoglobulin-associated alpha antibody|Ly54 antibody|MB 1 antibody|MB 1 membrane glycoprotein antibody|MB-1 membrane glycoprotein antibody|MB1 antibody|MB1 membrane glycoprotein antibody|Membrane bound immunoglobulin associated protein antibody|Membrane-bound immunoglobulin-associated protein antibody|Surface IgM associated protein antibody|Surface IgM-associated protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD79A antibody, ASA-B0401, Western blotting
All lanes: Anti CD79a (ASA-B0401) at 0.5ug/ml
WB: Recombinant human CD79a Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti CD79a (ASA-B0401) at 0.5ug/ml
WB: Recombinant human CD79a Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- CD79A antibody, ASA-B0401, Western blotting
All lanes: Anti CD79a (ASA-B0401) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Predicted bind size: 25KD
Observed bind size: 37KD
All lanes: Anti CD79a (ASA-B0401) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Predicted bind size: 25KD
Observed bind size: 37KD
Anti- CD79A antibody, ASA-B0401, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue