More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD68 molecule | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CD68(341-354aa FCIIRRRPSAYQAL). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD68 |
Protein | Macrosialin |
Uniprot ID | P34810 |
Function | Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. |
Tissue Specificity | Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. |
Sub-cellular localization | Isoform Short: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the LAMP family. |
Aliases | CD 68 antibody|CD68 antibody|CD68 antigen antibody|CD68 molecule antibody|CD68_HUMAN antibody|DKFZp686M18236 antibody|GP110 antibody|LAMP4 antibody|Macrophage antigen CD68(microsialin) antibody|MACROPHAGE ANTIGEN CD68 antibody|macrosialin antibody|SCARD1 antibody|Scavenger receptor class D member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD68 antibody, ASA-B0400, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human CD68, 42.5KD(162aa tag+ T128-L354)
Lane 1: Recombinant Human CD68 Protein 10ng
Lane 2: Recombinant Human CD68 Protein 5ng
Lane 3: Recombinant Human CD68 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human CD68, 42.5KD(162aa tag+ T128-L354)
Lane 1: Recombinant Human CD68 Protein 10ng
Lane 2: Recombinant Human CD68 Protein 5ng
Lane 3: Recombinant Human CD68 Protein 2.5ng
Anti-CD68 antibody, ASA-B0400, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
