CD68 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
CD68 molecule	Polyclonal	IgG	Rabbit	Mouse, Rat	IHC-P, IHC-F, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse CD68(312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CD68
Protein	Macrosialin
Uniprot ID	P31996
Function	Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue Specificity	Expressed in tissue macrophages and to a lesser extent in dendritic cells.
Sub-cellular localization	Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.
Sequence Similarities	Belongs to the LAMP family.
Aliases	CD 68 antibody|CD68 antibody|CD68 antigen antibody|CD68 molecule antibody|CD68_HUMAN antibody|DKFZp686M18236 antibody|GP110 antibody|LAMP4 antibody|Macrophage antigen CD68(microsialin) antibody|MACROPHAGE ANTIGEN CD68 antibody|macrosialin antibody|SCARD1 antibody|Scavenger receptor class D member 1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Mouse, Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Mouse, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information