CD62P Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
selectin P(granule membrane protein 140kDa, antigen CD62)	Polyclonal	IgG	Rabbit	Human	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD62P(49-66aa KAYSWNISRKYCQNRYTD).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SELP
Protein	P-selectin
Uniprot ID	P16109
Function	Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
Tissue Specificity	Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Sub-cellular localization	Membrane; Single-pass type I membrane protein.
Sequence Similarities	Belongs to the selectin/LECAM family.
Aliases	Antigen CD62 antibody|CD62 antibody|CD62 antigen-like family member P antibody|CD62P antibody|CD62P antigen antibody|FLJ45155 antibody|GMP 140 antibody|GMP-140 antibody|GMP140 antibody|GMRP antibody|Granule membrane protein 140 antibody|Granule membrane protein 140kDa antibody|Granulocyte membrane protein antibody|GRMP antibody|LECAM 3 antibody|LECAM3 antibody|Leukocyte endothelial cell adhesion molecule 3 antibody|Leukocyte-endothelial cell adhesion molecule 3 antibody|LYAM3_HUMAN antibody|P Selectin antibody|P-selectin antibody|PADGEM antibody|Platelet activation dependent granule external membrane protein antibody|Platelet activation dependent granule-external membrane protein antibody|Platelet alpha granule membrane protein antibody|PSEL antibody|Selectin P(granule membrane protein 140kDa antigen CD62) antibody|Selectin P(granule membrane protein 140kDa, antigen CD62) antibody|SELP antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information