CD62P Antibody View larger

CD62P Antibody

ASA-B0395

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

selectin P(granule membrane protein 140kDa, antigen CD62) Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CD62P(49-66aa KAYSWNISRKYCQNRYTD).

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SELP

Protein

P-selectin

Uniprot ID

P16109

Function

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

Tissue Specificity

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Sub-cellular localization

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the selectin/LECAM family.

Aliases

Antigen CD62 antibody|CD62 antibody|CD62 antigen-like family member P antibody|CD62P antibody|CD62P antigen antibody|FLJ45155 antibody|GMP 140 antibody|GMP-140 antibody|GMP140 antibody|GMRP antibody|Granule membrane protein 140 antibody|Granule membrane protein 140kDa antibody|Granulocyte membrane protein antibody|GRMP antibody|LECAM 3 antibody|LECAM3 antibody|Leukocyte endothelial cell adhesion molecule 3 antibody|Leukocyte-endothelial cell adhesion molecule 3 antibody|LYAM3_HUMAN antibody|P Selectin antibody|P-selectin antibody|PADGEM antibody|Platelet activation dependent granule external membrane protein antibody|Platelet activation dependent granule-external membrane protein antibody|Platelet alpha granule membrane protein antibody|PSEL antibody|Selectin P(granule membrane protein 140kDa antigen CD62) antibody|Selectin P(granule membrane protein 140kDa, antigen CD62) antibody|SELP antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD62P antibody, ASA-B0395, Western blotting
All lanes: Anti CD62P (ASA-B0395) at 0.5ug/ml
WB: PANC Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 140KD
Anti- CD62P antibody, ASA-B0395, IHC(P)
IHC(P): Human Placenta Tissue
Anti- CD62P antibody, ASA-B0395, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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