More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| selectin P (granule membrane protein 140kDa, antigen CD62) | Polyclonal | IgG | Rabbit | Human | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CD62P recombinant protein (Position: W42-G271). Human CD62P shares 75.2% and 75.7% amino acid (aa) sequence identity with mouse and rat CD62P, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SELP |
Protein | P-selectin |
Uniprot ID | P16109 |
Function | Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. |
Tissue Specificity | Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the selectin/LECAM family. |
Aliases | Antigen CD62 antibody|CD62 antibody|CD62 antigen-like family member P antibody|CD62P antibody|GMP 140 antibody|GMP-140 antibody|GMP140 antibody|GMRP antibody|Granule membrane protein 140 antibody|Granulocyte membrane protein antibody|GRMP antibody|LECAM 3 antibody|LECAM3 antibody|Leukocyte endothelial cell adhesion molecule 3 antibody|Leukocyte-endothelial cell adhesion molecule 3 antibody|LYAM3_HUMAN antibody|P Selectin antibody|P-selectin antibody|PADGEM antibody|Platelet activation dependent granule-external membrane protein antibody|Platelet alpha granule membrane protein antibody|PSEL antibody|Selectin P (granule membrane protein 140kDa, antigen CD62) antibody|SELP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD62P antibody, ASA-B0396, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti- CD62P antibody, ASA-B0396, Western blotting
All lanes: Anti CD62P (ASA-B0396) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: K562 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 140KD
All lanes: Anti CD62P (ASA-B0396) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: K562 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 140KD
