More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| selectin L | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived human CD62L recombinant protein (Position: W39-Y372). Human CD62L shares 77.5 % and 78.7 % amino acid (aa) sequence identity with mouse and rat CD62L, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SELL |
Protein | L-selectin |
Uniprot ID | P14151 |
Function | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. |
Tissue Specificity | Expressed in B-cell lines and T-lymphocytes. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the selectin/LECAM family. |
Aliases | nephropathy, susceptibility to, included antibody|L Selectin antibody|L-selectin antibody|LAM-1 antibody|LAM1 antibody|LECAM1 antibody|LEU8 antibody| Leukocyte adhesion molecule 1 antibody|Leukocyte surface antigen Leu-8 antibody|Leukocyte surface antigen Leu8 antibody|Leukocyte-endothelial cell adhesion molecule 1 antibody|LNHR antibody|LSEL antibody|Ly-22 antibody|Ly-m22 antibody|Lyam-1 antibody|LYAM1 antibody|LYAM1_HUMAN antibody|Lymph node homing receptor antibody|Lymphocyte adhesion molecule 1 antibody| Lymphocyte antigen 22 antibody|Lymphocyte surface MEL-14 antigen antibody| MEL-14 antibody|Pln homing receptor antibody|PLNHR antibody|Selectin L antibody|Selectin, lymphocyte antibody|SELL antibody|TQ1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD62L antibody, ASA-B0394, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti- CD62L antibody, ASA-B0394, Western blotting
All lanes: Anti CD62L (ASA-B0394) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: HL60 Whole Cell Lysate at 40ug
Lane 5: Rat Spleen Tissue Lysate at 50ug
Lane 6: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 42KD
Observed bind size: 42KD
All lanes: Anti CD62L (ASA-B0394) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: HL60 Whole Cell Lysate at 40ug
Lane 5: Rat Spleen Tissue Lysate at 50ug
Lane 6: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 42KD
Observed bind size: 42KD
