More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| selectin E | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CD62E(33-50aa YDEASAYCQQRYTHLVAI), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SELE |
Protein | E-selectin |
Uniprot ID | P16581 |
Function | Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the selectin/LECAM family. |
Aliases | CD 62E antibody|CD26E antibody|CD62 antigen-like family member E antibody|CD62E antibody|CD62E antigen antibody|E selectin antibody|E-selectin antibody|ELAM 1 antibody|ELAM antibody|ELAM-1 antibody|ELAM1 antibody|Endothelial adhesion molecule 1 antibody|Endothelial leukocyte adhesion molecule 1 antibody|ESEL antibody|LECAM 2 antibody|LECAM2 antibody|Leukocyte endothelial cell adhesion molecule 2 antibody|Leukocyte-endothelial cell adhesion molecule 2 antibody|LYAM2_HUMAN antibody|SELE antibody|Selectin E antibody|SelectinE antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD62E antibody, ASA-B0392, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 1: PANC Cell Lysate
Lane 2: MM231 Cell Lysate
Anti-CD62E antibody, ASA-B0392, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
