More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD55 molecule, decay accelerating factor for complement (Cromer blood group) | Polyclonal | IgG | Rabbit | Human, Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD55 |
Protein | Complement decay-accelerating factor |
Uniprot ID | P08174 |
Function | This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. |
Tissue Specificity | Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. |
Sub-cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the receptors of complement activation (RCA) family. |
Aliases | CD 55 antibody|CD55 antibody|CD55 antigen antibody|CD55 Cromer blood group system antibody| CD55 molecule antibody|CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody|Cd55a antibody|Complement decay accelerating factor antibody| Complement decay-accelerating factor antibody|Complement decay-accelerating factor, GPI-anchored antibody|CR antibody|CROM antibody|Cromer Blood Group antigen antibody|Cromer blood group system antibody|DAF antibody|Daf-GPI antibody| DAF_HUMAN antibody|Daf1 antibody|Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody|Decay accelarating factor 1, isoform CRA_a antibody|Decay accelerating factor (GPI-form) antibody|Decay Accelerating Factor for Complement antibody|Decay accelerating factor GPI-form antibody|Decay accelerating factor soluble-form antibody|GPI-DAF antibody|TC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD55 antibody, ASA-B0390, Western blotting
All lanes: Anti CD55 (ASA-B0390) at 0.5ug/ml
WB: K562 Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti CD55 (ASA-B0390) at 0.5ug/ml
WB: K562 Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
Anti- CD55 antibody, ASA-B0390, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
