More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD47 molecule | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CD47(308-323aa EPLNAFKESKGMMNDE), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD47 |
Protein | Leukocyte surface antigen CD47 |
Uniprot ID | Q08722 |
Function | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. |
Tissue Specificity | Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | |
Aliases | Antigenic surface determinant protein OA3 antibody|CD 47 antibody|CD47 antibody|Cd47 antibody|CD47 antigen(Rh-related antigen, integrin-associated signal transducer) antibody|CD47 antigen antibody|CD47 glycoprotein antibody|CD47 molecule antibody|CD47_HUMAN antibody|IAP antibody|Integrin Associated Protein antibody|Integrin associated signal transducer antibody|Integrin-associated protein antibody|Leukocyte surface antigen CD47 antibody|MER 6 antibody|MER6 antibody|OA 3 antibody|OA3 antibody|OTTHUMP00000041152 antibody|OTTHUMP00000041153 antibody|Protein MER6 antibody|Rh related antigen antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD47 antibody, ASA-B0389, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-CD47 antibody, ASA-B0389, Western blotting
All lanes: Anti CD47 (ASA-B0389) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 35KD
Observed bind size: 35KD
All lanes: Anti CD47 (ASA-B0389) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 35KD
Observed bind size: 35KD
