More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| protein tyrosine phosphatase, receptor type, C | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CD45(1209-1227aa MVSTFEQYQFLYDVIASTY). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PTPRC |
Protein | Receptor-type tyrosine-protein phosphatase C |
Uniprot ID | P08575 |
Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Membrane. |
Sequence Similarities | Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. |
Aliases | B220 antibody|CD 45 antibody|CD45 antibody|CD45 antigen antibody|CD45R antibody|GP180 antibody|L CA antibody|L-CA antibody|LCA antibody|Leukocyte common antigen antibody|loc antibody|Ly-5 antibody|LY5 antibody|Lyt-4 antibody|OTTHUMP00000033813 antibody|OTTHUMP00000033816 antibody|OTTHUMP00000033817 antibody|OTTHUMP00000038574 antibody|Protein tyrosine phosphatase receptor type C antibody|Protein tyrosine phosphatase receptor type c polypeptide antibody|protein tyrosine phosphatase, receptor type, C antibody|PTPRC antibody|PTPRC_HUMAN antibody|Receptor-type tyrosine-protein phosphatase C antibody|SCID due to PTPRC deficiency antibody|T200 antibody|T200 glycoprotein antibody|T200 leukocyte common antigen antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD45 antibody, ASA-B0386, Western blotting
All lanes: Anti CD45 (ASA-B0386) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 147KD
Observed bind size: 147KD
All lanes: Anti CD45 (ASA-B0386) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 147KD
Observed bind size: 147KD
Anti-CD45 antibody, ASA-B0386, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti-CD45 antibody, ASA-B0386, ICC
ICC: JURKAT Cell
ICC: JURKAT Cell