CD45  Antibody View larger

CD45 Antibody

ASA-B0387

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

protein tyrosine phosphatase, receptor type, C Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human CD45 recombinant protein (Position: R1113-S1304). Human CD45 shares 68% amino acid (aa) sequence identity with both mouse and rat CD45.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

PTPRC

Protein

Receptor-type tyrosine-protein phosphatase C

Uniprot ID

P08575

Function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

Tissue Specificity

Sub-cellular localization

Membrane.

Sequence Similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.

Aliases

B220 antibody|CD 45 antibody|CD45 antibody|CD45 antigen antibody|CD45R antibody|GP180 antibody|L CA antibody|L-CA antibody|LCA antibody|Leukocyte common antigen antibody|loc antibody|Ly-5 antibody|LY5 antibody|Ly5, homolog of antibody|Lyt-4 antibody|OTTHUMP00000033813 antibody|OTTHUMP00000033816 antibody|OTTHUMP00000033817antibody|OTTHUMP00000038574 antibody|Protein tyrosine phosphatase receptor type C antibody|Protein tyrosine phosphatase receptor type c polypeptide antibody|protein tyrosine phosphatase, receptor type, C antibody|PTPRC antibody|PTPRC_HUMAN antibody|Receptor-type tyrosine-protein phosphatase C antibody|SCID due to PTPRC deficiency antibody|T200 antibody|T200 glycoprotein antibody|T200 leukocyte common antigen antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD45 antibody, ASA-B0387--1.jpg
All lanes: Anti CD45 (ASA-B0387) at 0.5ug/ml
WB: Recombinant Human CD45 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti-CD45 antibody, ASA-B0387--2.jpg
All lanes: Anti CD45 (ASA-B0387) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 147KD
Observed bind size: 147KD
Anti-CD45 antibody, ASA-B0387--3.jpg
IHC(P): Human Tonsil Tissue

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