More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD44 molecule (Indian blood group) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CD44(29-56aa RFAGVFHVEKNGRYSISRTEAADLCKAF), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD44 |
Protein | CD44 antigen |
Uniprot ID | P16070 |
Function | Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. |
Tissue Specificity | Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Note: Colocalizes with actin in membrane protrusions at wounding edges. |
Sequence Similarities | Contains 1 Link domain. |
Aliases | LHR antibody|BA-1 antibody|CD 44 antibody|CD44 antibody|CD44 antigen antibody|CD44 isoform RC antibody|CD44 molecule (Indian blood group) antibody|CD44 molecule antibody|CD44_HUMAN antibody|CDw44 antibody|Cell surface glycoprotein CD44 antibody|chondroitin sulfate proteoglycan 8 antibody|CSPG8 antibody|ECM III antibody|ECMR III antibody|ECMR-III antibody|ECMRIII antibody|Epican antibody|Extracellular Matrix Receptor III antibody|GP90 Lymphocyte Homing Adhesion Receptor antibody|GP90 lymphocyte homing/adhesion receptor antibody|HCAM antibody|HCELL antibody|hematopoietic cell E- and L-selectin ligand antibody|Heparan Sulfate Proteoglycan antibody|Hermes Antigen antibody|homing function and Indian blood group system antibody|HSA antibody|HUTCH I antibody|HUTCH-I antibody|HUTCH1 antibody|Hyaluronate Receptor antibody|IN antibody|Indian blood group antibody|Inlu Related p80 Glycoprotein antibody|lhr antibody|Ly 24 antibody|MC56 antibody|MDU2 antibody|MDU3 antibody|MGC10468 antibody|MIC4 antibody|MUTCH I antibody|MUTCH1 antibody|PGP 1 antibody|PGP-1 antibody|PGP-I antibody|PGP1 antibody|Phagocytic Glycoprotein 1 antibody|Phagocytic glycoprotein I antibody|Transmembrane glycoprotein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD44 antibody, ASA-B0385, Western blotting
All lanes: Anti CD44 (ASA-B0385) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 115KD
All lanes: Anti CD44 (ASA-B0385) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 115KD
Anti- CD44 antibody, ASA-B0385, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
