More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sialophorin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SPN |
Protein | Leukosialin |
Uniprot ID | P16150 |
Function | One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). |
Tissue Specificity | Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | |
Aliases | CD 43 antibody|CD43 antibody|CD43 antigen antibody|Galactoglycoprotein antibody|Galactoglycoprotein antibody|GALGP antibody|GPL 115 antibody|GPL115 antibody|Human gene for sialophorin antibody|Leucocyte sialoglycoprotein antibody|Leucocyte sialoglycoprotein antibody|LEUK_HUMAN antibody|Leukocyte sialoglycoprotein antibody|Leukosialin antibody|LSN antibody|Sialophorin (leukosialin) antibody|Sialophorin antibody|SPN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD43 antibody, ASA-B0383, Western blotting
All lanes: Anti CD43 (ASA-B0383) at 0.5ug/ml
WB: Recombinant Human CD43 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti CD43 (ASA-B0383) at 0.5ug/ml
WB: Recombinant Human CD43 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti-CD43 antibody, ASA-B0383, IHC(P)
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue
Anti-CD43 antibody, ASA-B0383, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue