More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD40 ligand | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CD40L(47-65aa HRRLDKIEDERNLHEDFVF), different from the related mouse sequence by three amino acids, rat sequence by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD40LG |
Protein | CD40 ligand(CD40-L) |
Uniprot ID | P29965 |
Function | Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. |
Tissue Specificity | Specifically expressed on activated CD4+ T- lymphocytes. |
Sub-cellular localization | Cell membrane; Single-pass type II membrane protein. |
Sequence Similarities | Belongs to the tumor necrosis factor family. |
Aliases | CD 154 antibody|CD 40L antibody|CD154 antibody|CD154 antigen antibody|CD40 antigen ligand antibody|CD40 homologue antibody|CD40 L antibody|CD40 ligand antibody|CD40 ligand, soluble form antibody|CD40-L antibody|CD40L_HUMAN antibody|CD40LG antibody|gp39 antibody|hCD40L antibody|HIGM1 antibody|Hyper IgM syndrome antibody|IGM antibody|IMD3 antibody|Ly62 antibody|T B cell activating molecule antibody|T BAM antibody|T cell antigen GP39 antibody|T-cell antigen Gp39 antibody|TBAM antibody|TNF related activation protein antibody|TNF superfamily member 5 antibody|TNF-related activation protein antibody|Tnfsf5 antibody|TRAP antibody|Tumor Necrosis Factor(ligand) Superfamily Member 5 antibody|Tumor necrosis factor ligand superfamily member 5 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD40Lantibody, ASA-B0382, Western blotting
All lanes: Anti CD40L (ASA-B0382) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HMY2 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
All lanes: Anti CD40L (ASA-B0382) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HMY2 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
Anti CD40Lantibody, ASA-B0382 IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
