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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD34 molecule | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CD34(366-382aa QATSRNGHSARQHVVAD), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD34 |
Protein | Hematopoietic progenitor cell antigen CD34 |
Uniprot ID | P28906 |
Function | Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. |
Tissue Specificity | Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the CD34 family. |
Aliases | CD34 antibody|Cd34 antibody|CD34 antigen antibody|CD34 molecule antibody|CD34_HUMAN antibody|Cluster designation 34 antibody|Hematopoietic progenitor cell antigen CD34 antibody|HPCA1 antibody|Mucosialin antibody|OTTHUMP00000034733 antibody|OTTHUMP00000034734 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD34 antibody, ASA-B0376, Western blotting
Lane 1: Recombinant Human CD34 Protein 10ng
Lane 2: Recombinant Human CD34 Protein 5ng
Lane 3: Recombinant Human CD34 Protein 2.5ng
Lane 1: Recombinant Human CD34 Protein 10ng
Lane 2: Recombinant Human CD34 Protein 5ng
Lane 3: Recombinant Human CD34 Protein 2.5ng
Anti-CD34 antibody, ASA-B0376, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
Anti-CD34 antibody, ASA-B0376, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue