More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| platelet/endothelial cell adhesion molecule 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PECAM1 |
Protein | Platelet endothelial cell adhesion molecule |
Uniprot ID | P16284 |
Function | Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). |
Tissue Specificity | Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level). |
Sub-cellular localization | Isoform Long: Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction. Note: Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. |
Sequence Similarities | Contains 6 Ig-like C2-type (immunoglobulin-like) domains. |
Aliases | Adhesion molecule antibody|CD31 antibody|CD31 antigen antibody|CD31 EndoCAM antibody|Endocam antibody|FLJ34100 antibody|FLJ58394 antibody|GPIIA antibody|GPIIA' antibody|PECA1 antibody|PECA1_HUMAN antibody|Pecam 1 antibody|PECAM 1 CD31 EndoCAM antibody|PECAM antibody|PECAM-1 antibody|Pecam1 antibody|Platelet endothelial cell adhesion molecule antibody|Platelet/endothelial cell adhesion molecule 1 antibody|Platelet/endothelial cell adhesion molecule antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD31 antibody, ASA-B0375--1.jpg
All lanes: Anti CD31 (ASA-B0375) at 0.5ug/ml
WB: Recombinant Human CD31 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti CD31 (ASA-B0375) at 0.5ug/ml
WB: Recombinant Human CD31 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-CD31 antibody, ASA-B0375--2.jpg
All lanes: Anti CD31 (ASA-B0375) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U937 Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
All lanes: Anti CD31 (ASA-B0375) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U937 Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
Anti-CD31 antibody, ASA-B0375-4.JPG
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue