More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD3e molecule, epsilon (CD3-TCR complex) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD3E |
Protein | T-cell surface glycoprotein CD3 epsilon chain |
Uniprot ID | P07766 |
Function | The CD3 complex mediates signal transduction. |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 Ig-like (immunoglobulin-like) domain. |
Aliases | CD3 epsilon antibody|CD3e antibody|CD3E antibody|CD3e antigen epsilon polypeptide (TiT3 complex) antibody|CD3E antigen epsilon polypeptide antibody|CD3E antigen, epsilon subunit antibody|CD3e molecule epsilon antibody|CD3e molecule, epsilon (CD3 TCR complex) antibody|CD3e molecule, epsilon (CD3-TCR complex) antibody|CD3E_HUMAN antibody|IMD18 antibody|T cell antigen receptor complex epsilon subunit of T3 antibody|T cell surface antigen T3/Leu 4 epsilon chain antibody|T cell surface glycoprotein CD3 epsilon chain antibody|T-cell surface antigen T3/Leu-4 epsilon chain antibody|T-cell surface glycoprotein CD3 epsilon chain antibody|T3E antibody|TCRE antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD3 epsilon antibody, ASA-B0371--1.jpg
All lanes: Anti CD3 Epsilon (ASA-B0371) at 0.5ug/ml
WB : Recombinant Human CD3epsilon Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti CD3 Epsilon (ASA-B0371) at 0.5ug/ml
WB : Recombinant Human CD3epsilon Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-CD3 epsilon antibody, ASA-B0371--2.jpg
All lanes: Anti CD3 Epsilon (ASA-B0371) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti CD3 Epsilon (ASA-B0371) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
Anti-CD3 epsilon antibody, ASA-B0371--3.jpg
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue