CD22 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
CD22 molecule	Polyclonal	IgG	Rabbit	Human	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD22(832-847aa ERPQAQENVDYVILKH).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CD22
Protein	B-cell receptor CD22
Uniprot ID	P20273
Function	Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
Tissue Specificity	B-lymphocytes.
Sub-cellular localization	Cell membrane; Single-pass type I membrane protein.
Sequence Similarities	Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Aliases	B cell receptor CD22 precursor antibody|B lymphocyte cell adhesion molecule antibody|B-cell receptor CD22 antibody|B-lymphocyte cell adhesion molecule antibody|BL CAM antibody|BL-CAM antibody|BLCAM antibody|CD 22 antibody|CD22 antibody|CD22 antigen antibody|CD22 molecule antibody|CD22 protein antibody|CD22_HUMAN antibody|Lectin 2 antibody|Leu14 antibody|Lyb8 antibody|MGC130020 antibody|sialic acid binding Ig like lectin 2 antibody|Sialic acid binding immunoglobulin like lectin 2 antibody|Sialic acid-binding Ig-like lectin 2 antibody|SIGLEC 2 antibody|Siglec-2 antibody|SIGLEC2 antibody|T cell surface antigen Leu 14 antibody|T-cell surface antigen Leu-14 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information