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CD18 Antibody

ASA-B0359

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
integrin, beta 2(complement component 3 receptor 3 and 4 subunit) Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD18(732-748aa LREYRRFEKEKLKSQWN), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 ITGB2

Protein

 Integrin beta-2

Uniprot ID

 P05107

Function

 Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation.

Tissue Specificity

Sub-cellular localization

 Membrane; Single-pass type I membrane protein.

Sequence Similarities

 Belongs to the integrin beta chain family.

Aliases

 95 subunit beta antibody|Beta 2(antigen CD18(p95) antibody|CD 18 antibody|CD18 antibody|CD18 antigen antibody|Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody|Cell surface adhesion glycoproteins LFA 1/CR3/p150 95 subunit beta antibody|Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody|Cell surface adhesion glycoproteins LFA 1/CR3/p15095 beta subunit antibody|Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody|Complement component 3 receptor 3 and 4 subunit antibody|Complement receptor C3 beta subunit antibody|Complement receptor C3 subunit beta antibody|Integrin beta 2 antibody|Integrin beta chain beta 2 antibody|Integrin beta-2 antibody|Integrin, beta 2(complement component 3 receptor 3 and 4 subunit) antibody|ITB2_HUMAN antibody|ITGB 2 antibody|ITGB2 antibody|LAD antibody|LCAMB antibody|Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody|Leukocyte Cell Adhesion Molecule CD18 antibody|LFA 1 antibody|LFA1 antibody|Lymphocyte function associated antigen 1 antibody|MAC 1 antibody|MAC1 antibody|Macrophage antigen 1(mac1) beta subunit antibody|MF17 antibody|MFI7 antibody|OTTHUMP00000115278 antibody|OTTHUMP00000115279 antibody|OTTHUMP00000115280 antibody|OTTHUMP00000115281 antibody|OTTHUMP00000115282 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD18 antibody, ASA-B0359, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: HELA Cell Lysate
Anti-CD18 antibody, ASA-B0359, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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