More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| endoglin | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CD105(26-44aa ETVHCDLQPVGPERGEVTY). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ENG |
Protein | Endoglin |
Uniprot ID | P17813 |
Function | Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. |
Tissue Specificity | Endoglin is restricted to endothelial cells in all tissues except bone marrow. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | |
Aliases | CD 105 antibody|CD105 antibody|CD105 antigen antibody|EGLN_HUMAN antibody|END antibody|Endoglin antibody|ENG antibody|FLJ41744 antibody|HHT1 antibody|ORW antibody|ORW1 antibody|Osler Rendu Weber syndrome 1 antibody|RP11 228B15.2 antibody|SN6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD105 antibody, ASA-B0341, Western blotting
Lane 1: Recombinant Human CD105 Protein 10ng
Lane 2: Recombinant Human CD105 Protein 5ng
Lane 3: Recombinant Human CD105 Protein 2.5ng
Lane 1: Recombinant Human CD105 Protein 10ng
Lane 2: Recombinant Human CD105 Protein 5ng
Lane 3: Recombinant Human CD105 Protein 2.5ng
Anti-CD105 antibody, ASA-B0341, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
