CBS Antibody View larger

CBS Antibody

ASA-B0325

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

cystathionine-beta-synthase Polyclonal IgG Rabbit Human, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human CBS recombinant protein (Position: A331-K551). Human CBS shares 83% amino acid (aa) sequence identity with both mouse and rat CBS.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

CBS

Protein

Cystathionine beta-synthase

Uniprot ID

P35520

Function

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity).

Tissue Specificity

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Belongs to the cysteine synthase/cystathionine beta- synthase family.

Aliases

AI047524 antibody|AI303044 antibody|Beta thionase antibody|Beta-thionase antibody|Cbs antibody|Cbs cystathionine beta-synthase antibody|CBS_HUMAN antibody|Cystathionine beta synthase antibody|Cystathionine beta-synthase antibody|EC 4.2.1.22 antibody|HIP 4 antibody|HIP4 antibody|Methylcysteine synthase antibody|MGC18856 antibody|MGC18895 antibody|MGC37300 antibody|OTTHUMP00000109416 antibody|OTTHUMP00000109418 antibody|Serine sulfhydrase antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CBS antibody, ASA-B0325, Western blotting
All lanes: Anti CBS (ASA-B0325) at 0.5ug/ml
WB: Recombinant human CBS Protein 0.5ng
Predicted bind size: 42KD
Observed bind size: 42KD
Anti- CBS antibody, ASA-B0325, Western blotting
All lanes: Anti CBS (ASA-B0325) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Hela Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: Hepg2 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
Anti- CBS antibody, ASA-B0325, IHC(P)
IHC(P): Human Liver Cancer Tissue

© 2024 Novateinbio.com