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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caveolin 2 | Polyclonal | IgG | Rabbit | Human, Mouse | IF, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Caveolin-2 recombinant protein (Position: M1-D162). Human Caveolin-2 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat Caveolin-2, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CAV2 |
Protein | Caveolin-2 |
Uniprot ID | P51636 |
Function | May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity). |
Tissue Specificity | Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts. |
Sub-cellular localization | Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Note: Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27- phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. |
Sequence Similarities | Belongs to the caveolin family. |
Aliases | CAV antibody|CAV2 antibody|CAV2_HUMAN antibody|Caveolae protein 20 kD antibody|Caveolae protein 20kD antibody|Caveolin 2 isoform a and b antibody|Caveolin 2 isoform c antibody|Caveolin-2 antibody|Caveolin2 antibody|MGC12294 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Caveolin-2 antibody, ASA-B0321, Western blotting
All lanes: Anti Caveolin-2 (ASA-B0321) at 0.5ug/ml
WB: Recombinant Human Caveolin-2 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti Caveolin-2 (ASA-B0321) at 0.5ug/ml
WB: Recombinant Human Caveolin-2 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti- Caveolin-2 antibody, ASA-B0321, Western blotting
All lanes: Anti Caveolin-2 (ASA-B0321) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Predicted bind size: 17KD
Observed bind size: 17KD
All lanes: Anti Caveolin-2 (ASA-B0321) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Predicted bind size: 17KD
Observed bind size: 17KD
Anti- Caveolin-2 antibody, ASA-B0321,IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue