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Caveolin-1 Antibody View larger

Caveolin-1 Antibody

ASA-B0319

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
caveolin 1, caveolae protein, 22kDa Polyclonal IgG Rabbit Human, Mouse IHC-P, IHC-F, ICC, WB Immunogen affinity purified.

Immunogen

E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Caveolin-1, respectively.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CAV1

Protein

 Caveolin-1

Uniprot ID

 Q03135

Function

 May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.

Tissue Specificity

Expressed in muscle and lung, less so in liver, brain and kidney.

Sub-cellular localization

 Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts. Potential hairpin- like structure in the membrane. Membrane protein of caveolae.

Sequence Similarities

 Belongs to the caveolin family.

Aliases

 BSCL3 antibody|CAV 1 antibody|CAV antibody|CAV1 antibody|CAV1_HUMAN antibody|caveolae protein, 22 kD antibody|caveolin 1 alpha isoform antibody|caveolin 1 beta isoform antibody|Caveolin 1 caveolae protein 22kDa antibody|Caveolin-1 antibody|Caveolin1 antibody|cell growth-inhibiting protein 32 antibody|CGL3 antibody|LCCNS antibody|MSTP085 antibody|OTTHUMP00000025031 antibody|PPH3 antibody|VIP 21 antibody|VIP 21 antibody|VIP21 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Human, MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Caveolin-1 antibody, ASA-B0319, Western blotting
All lanes: Anti Caveolin-1 (ASA-B0319) at 0.5ug/ml
WB: Recombinant Human Caveolin-1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti- Caveolin-1 antibody, ASA-B0319, Western blotting
All lanes: Anti Caveolin-1 (ASA-B0319) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
Anti- Caveolin-1 antibody, ASA-B0319, IHC(P)
IHC(P): Human Placenta Tissue

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