Cathepsin D Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
cathepsin D	Polyclonal	IgG	Rabbit	Human	IHC-P, WB	Immunogen affinity purified.
Immunogen
E.coli-derived human Cathepsin D recombinant protein (Position: G65-L412). Human Cathepsin D shares 85% amino acid (aa) sequence identity with both mouse and rat Cathepsin D.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CTSD
Protein	Cathepsin D
Uniprot ID	P07339
Function	Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Tissue Specificity	Expressed in the aorta extrcellular space (at protein level).
Sub-cellular localization	Lysosome. Melanosome. Secreted, extracellular space. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
Sequence Similarities	Belongs to the peptidase A1 family.
Aliases	CatD antibody|CATD_HUMAN antibody|Cathepsin D heavy chain antibody|Cathepsin D light chain antibody|CathepsinD antibody|CD antibody|Ceroid lipofuscinosis neuronal 10 antibody|CLN10 antibody|CPSD antibody|CTSD antibody|Lysosomal aspartyl peptidase antibody|Lysosomal aspartyl protease antibody|MGC2311 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information