More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cathepsin D | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Cathepsin D recombinant protein (Position: G65-L412). Human Cathepsin D shares 85% amino acid (aa) sequence identity with both mouse and rat Cathepsin D. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CTSD |
Protein | Cathepsin D |
Uniprot ID | P07339 |
Function | Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. |
Tissue Specificity | Expressed in the aorta extrcellular space (at protein level). |
Sub-cellular localization | Lysosome. Melanosome. Secreted, extracellular space. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). |
Sequence Similarities | Belongs to the peptidase A1 family. |
Aliases | CatD antibody|CATD_HUMAN antibody|Cathepsin D heavy chain antibody|Cathepsin D light chain antibody|CathepsinD antibody|CD antibody|Ceroid lipofuscinosis neuronal 10 antibody|CLN10 antibody|CPSD antibody|CTSD antibody|Lysosomal aspartyl peptidase antibody|Lysosomal aspartyl protease antibody|MGC2311 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cathepsin D antibody, ASA-B0312--1.jpg
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-Cathepsin D antibody, ASA-B0312--2.jpg
All lanes: Anti-Cathepsin D(ASA-B0312) at 0.5ug/ml
WB: Recombinant Human Cathepsin D Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti-Cathepsin D(ASA-B0312) at 0.5ug/ml
WB: Recombinant Human Cathepsin D Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
