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Caspase-7(P11) Antibody View larger

Caspase-7(P11) Antibody

ASA-B0301

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
caspase 7, apoptosis-related cysteine peptidase Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11)(237-255aa RGSWFVQALCSILEEHGKD), different from the related mouse and rat sequences by two amino acids.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CASP7

Protein

 Caspase-7(CASP-7)

Uniprot ID

 P55210

Function

 Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-

Tissue Specificity

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Sub-cellular localization

 Cytoplasm.

Sequence Similarities

Aliases

 Apoptosis related cysteine peptidase antibody|Apoptosis Related Cysteine Protease antibody|Apoptotic protease Mch-3 antibody|Apoptotic Protease MCH3 antibody|CASP 7 antibody|CASP-7 antibody|CASP7 antibody|Caspase-7 subunit p11 antibody|Caspase7 antibody|CMH 1 antibody|CMH-1 antibody|CMH1 antibody|ICE LAP3 antibody|ICE like Apoptotic Protease 3 antibody|ICE-LAP3 antibody|ICE-like apoptotic protease 3 antibody|MCH3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Caspase-7(P11) antibody, ASA-B0301, Western blotting
All lanes: Anti CASP7(p11) (ASA-B0301) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 34KD
Observed bind size: 34KD
Anti-Caspase-7(P11) antibody, ASA-B0301, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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