More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caspase 7, apoptosis-related cysteine peptidase | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CASP7 recombinant protein (Position: A117-D198). Human CASP7 shares 92.7% amino acid (aa) sequence identity with both mouse and rat CASP7. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CASP7 |
Protein | Caspase-7 |
Uniprot ID | P55210 |
Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- |
Tissue Specificity | Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | Apoptosis related cysteine peptidase antibody|Apoptosis Related Cysteine Protease antibody|Apoptosis Related Cysteine Protease antibody|Apoptotic protease Mch-3 antibody|Apoptotic Protease MCH3 antibody|Apoptotic Protease MCH3 antibody|CASP 7 antibody|CASP-7 antibody|CASP7 antibody| CASP7_ HUMAN antibody|Caspase 7 antibody|Caspase 7 apoptosis related cysteine peptidase antibody|Caspase-7 subunit p11 antibody|Caspase7 antibody|CMH 1 antibody|CMH-1 antibody|CMH1 antibody|CMH1 antibody|ICE LAP3 antibody|ICE like Apoptotic Protease 3 antibody|ICE-LAP3 antibody|ICE-like apoptotic protease 3 antibody|LICE2 antibody|MCH3 antibody|MCH3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Caspase-7 antibody, ASA-B0300, Western blotting
All lanes: Anti Caspase-7(ASA-B0300) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Predicted bind size: 34KD
Observed bind size: 34KD
All lanes: Anti Caspase-7(ASA-B0300) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Predicted bind size: 34KD
Observed bind size: 34KD
Anti- Caspase-7 antibody, ASA-B0300,IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
