More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caspase 6, apoptosis-related cysteine peptidase | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of rat Caspase-6(P18)(7-27aa FYRSREVLDPAEQYKMDHKRR), different from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CASP6 |
Protein | Caspase-6 |
Uniprot ID | O35397 |
Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm . |
Sequence Similarities | |
Aliases | Apoptosis Related Cysteine Protease antibody|Apoptotic cysteine protease MCH2 antibody|Apoptotic protease MCH 2 antibody|Apoptotic protease Mch-2 antibody|Apoptotic protease MCH2 antibody|Casp 6 antibody|CASP-6 antibody|Casp6 antibody|Caspase 6 antibody|Caspase 6 apoptosis related cysteine protease antibody|Caspase 6 precursor antibody|Caspase 6, apoptosis related cysteine peptidase antibody|Caspase-6 subunit p18 antibody|Caspase6 antibody|Human cysteine protease Mch2 isoform alpha antibody|Mch 2 antibody|Mch2 antibody|OTTHUMP00000162742 antibody|OTTHUMP00000162743 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
-antibody-ASA-B0298-1.jpg)
Anti-Caspase-6(P18) antibody, ASA-B0298, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: NRK Cell Lysate
Lane 5: Mouse Liver Tissue Lysate
Lane 6: Mouse Kidney Tissue Lysate
Lane 7: Mouse Testis Tissue Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: NRK Cell Lysate
Lane 5: Mouse Liver Tissue Lysate
Lane 6: Mouse Kidney Tissue Lysate
Lane 7: Mouse Testis Tissue Lysate
-antibody-ASA-B0298-2.jpg)
Anti-Caspase-6(P18) antibody, ASA-B0298, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue
-antibody-ASA-B0298-3.jpg)
Anti-Caspase-6(P18) antibody, ASA-B0298, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue